The PEPFAR gender technical exchange, convened by the PEPFAR Gender Technical Working Group in collaboration with AIDSTAR-One, aimed to support the expansion of gender integration into PEPFAR programs. In response to the call for improved gender integration and mainstreaming, and through the Gender Challenge Fund and Gender-Based Violence Initiative, PEPFAR country teams are expanding their gender programming within HIV prevention, care, and treatment portfolios. Country teams requested an opportunity to come together for a technical update on gender and HIV within the context of PEPFAR, share successes and challenges in implementation, and build capacity to better plan and manage a strategic gender program portfolio. In response to this request, the PEPFAR Gender Technical Working Group and AIDSTAR-One held this PEPFAR gender technical exchange; this report provides highlights and themes from that exchange.